PepSep Series
Description
Experience the true potential of your proteomics applications with our high-performance liquid chromatography C18 reversed phase columns. PepSep columns are created with industry leading materials and connections to accommodate all your chromatographic separation needs. Discover the perfect solution for your Proteomics applications within our diverse PepSep column portfolio. With a wide range of columns available, featuring different lengths, inner diameters, and particle sizes, we are determined to meet your unique needs. Discover the perfect column for your specific requirements and revolutionize your proteomics analysis today.
Features
- patented female ultra-high pressure (UHP) connector for smooth, safe, and easy connections to the high-pressure end of the column (often via transfer line)
- UHP connection is tested to be used up to 1000 bar
- patented nanoConnect (nC) connector on the low-pressure end of the column makes union and emitter connections quick, reliable, and straightforward
- nC connector ensures a zero dead volume connection
Application
Part Number Material Description Categories Application recommendations Recommended Flow Rates 1895838 PepSep C18 40 cm x 150 µm, 1.5 µm Optimization General Proteomics, Plasma Proteomics, Mixed Samples, Gradient Versatility 500-1000 nL/min 1893479 PepSep C18 25 cm x 150 µm, 1.9 µm Optimization General Proteomics, Plasma Proteomics, Mixed Samples, Gradient Versatility 500-2000 nL/min 1893471 PepSep C18 15 cm x 150 µm, 1.9 µm Optimization General Proteomics, Plasma Proteomics, Mixed Samples, Gradient Versatility 500-2000 nL/min 1893474 PepSep C18 15 cm x 150 µm, 1.5 µm Optimization General Proteomics, Plasma Proteomics, Mixed Samples, Gradient Versatility 500-1500 nL/min 1895806 PepSep C18 15 cm x 100 µm, 3 µm Optimization Qualification of HPLC 500-4000 nL/min 1893480 PepSep C18 8 cm x 100 µm, 3 µm Optimization Qualification of HPLC 500-5000 nL/min 1893477 PepSep C18 25cm x 75 µm, 1.9 µm High-Sensitivity Single Cell Proteomics, Immunopeptidomics, General Proteomics 100-600 nL/min 1893627 PepSep C18 25cm x 50 µm, 1.5 µm High-Sensitivity Single Cell Proteomics, Immunopeptidomics, General Proteomics 50-200 nL/min 1893473 PepSep C18 15cm x 75 µm, 1.9 µm High-Sensitivity Single Cell Proteomics, Immunopeptidomics, General Proteomics 200-1000 nL/min 1893625 PepSep C18 15cm x 75 µm, 1.5 µm High-Sensitivity Single Cell Proteomics, Immunopeptidomics, General Proteomics 150-750 nL/min 1893626 PepSep C18 15cm x 50 µm, 1.5 µm High-Sensitivity Single Cell Proteomics, Immunopeptidomics, General Proteomics 50-300 nL/min 1895803 PepSep C18 10cm x 75 µm, 1.5 µm High-Sensitivity Single Cell Proteomics, Immunopeptidomics, General Proteomics 150-1000 nL/min 1893472 PepSep C18 10cm x 75 µm, 1.9 µm High-Sensitivity Single Cell Proteomics, Immunopeptidomics, General Proteomics 150-1500 nL/min 1893470 PepSep C18 8cm x 150 µm, 1.5 µm High-Throughput General Proteomics, Plasma Proteomics, Mixed Samples, Gradient Versatility, High-Throughput 500-5000 nL/min 1895619 PepSep C18 4cm x 150 µm, 1.9 µm High-Throughput General Proteomics, Plasma Proteomics, Mixed Samples, Gradient Versatility, High-Throughput 500-5000 nL/min *Optimization: Balance between Sensitivity & Throughput
Delivery Scope
Column
Technical Data
Stationary phase ReproSil Saphir C18 ReproSil-Pur C18-AQ ReproSil-Pur C18-AQ Particle size 1.5 µm 1.9 µm 3 µm Pore size 100 Å 120 Å 120 Å Surface area 400 m²/g 300 m²/g 300 m²/g pH range 2-9 2-8 2-8 Length 8, 10, 15, 25, 40, 50 cm 4, 10, 15, 25 cm 8, 15 cm Inner diameter 50, 75, 150 µm 75, 150 µm 100 µm Column type RP RP RP Max. Temperature 60 °C 60 °C 60 °C Max. Pressure 1000 bar 1000 bar 1000 bar Inlet UHP UHP UHP Outlet nanoConnect nanoConnect nanoConnect