PepSep Twenty-five Columns have a length of 25 cm. These columns would be an excellent choice for routine analysis requiring high sensitivity.
PepSep Twenty-five Columns are available in different inner diameters, particle sizes and inlets. The columns with a wider ID are a good choice to reduce backpressure, which can accelerate HPLC valve wear, overpressure HPLC systems and potentially cause column damage.
For smooth, safe, and easy connections to the high-pressure end of the column (often via transfer line) the columns are equipped with different connectors:
- patented female ultra-high pressure (UHP) connector. The UHP connection is tested to be used up to 1000 bar.
- or PepSeal connector. It is a 10-32 thread female union which enables easy, precise and fast connection to most LC systems with 1/16 connectors.
On the columns low pressure end, the columns are equipped with the patented nanoConnect (nC) connector, ensuring a zero dead volume connection. By design, the nanoConnect system makes union and emitter connections quick, reliable, and straightforward.
| Stationary phase | C18 Sapphir | C18 ReproSil AQ | C18 ReproSil AQ |
| Particle size | 1.5 µm | 1.9 µm | 3.0 µm |
| Pore size | 100 Å | 120 Å | 120 Å |
| Surface area | 400 m²/g | 300 m²/g | 300 m²/g |
| pH range | 2-9 | 2-8 | 2-8 |
| Length | 250 mm | ||
| Inner diameter | 50, 75, 100, 150 or 200 µm | ||
| Column type | RP | ||
| Max. Temperature | 60 °C | ||
| Max. Pressure | 1000 bar | ||
| Inlet | UHP or PepSeal | ||
| Outlet | nanoConnect | ||
