10 x RT-IC running buffer 1 (RB1)

50 mL of: 1.4 M NaCl, 27 mM KCl, 15 mM KH2PO4, 80 mM Na2HPO4, 0.1 % Pluronic

Yields 500 mL of: 140 mM NaCl, 2.7 mM KCl, 1.5 mM KH2PO4, 8 mM Na2HPO4, 0.01 % Pluronic.

Without Ca2+ or Mg2+.