Sample Buffer

50 ml of: 100 mM Hepes, 1.4 M NaCl, 0.5 % Tween20, 0.5 mM EDTA, 0.5 mM EGTA;
Yields 500mL of: 10 mM Hepes, 140 mM NaCl, 0.05 % Tween20, 50 µM EDTA, 50 µM EGTA