PepSep Fifteen Columns have a length of 15 cm. As a shorter column, faster separation gradients can be used, yielding results up to three times faster than columns used in standard proteomics workflows. This column would be an excellent choice for high-throughput applications.
PepSep Fifteen Columns are available in different widths or inner diameters. The column with a wider ID is a good choice to reduce backpressure, which can accelerate HPLC valve wear, overpressure HPLC systems and potentially cause column damage.
For smooth, safe, and easy connections to the high-pressure end of the column (often via transfer line) the columns are equipped with different connectors:
- patented female ultra-high pressure (UHP) connector. The UHP connection is tested to be used up to 1000 bar.
- or PepSeal connector. It is a 10-32 thread female union which enables easy, precise and fast connection to most LC systems with 1/16 connectors.
On the columns low pressure end, the columns are equipped with the patented nanoConnect (nC) connector, ensuring a zero dead volume connection. By design, the nanoConnect system makes union and emitter connections quick, reliable, and straightforward.
| Stationary phase | C18 Sapphir | C18 ReproSil AQ | C18 ReproSil AQ |
| Particle size | 1.5 µm | 1.9 µm | 3.0 µm |
| Pore size | 100 Å | 120 Å | 120 Å |
| Surface area | 400 m²/g | 300 m²/g | 300 m²/g |
| pH range | 2-9 | 2-8 | 2-8 |
| Length | 150 mm | ||
| Inner diameter | 50, 75, 100, 150 or 200 µm | ||
| Column type | RP | ||
| Max. Temperature | 60 °C | ||
| Max. Pressure | 1000 bar | ||
| Inlet | UHP or PepSeal | ||
| Outlet | nanoConnect | ||
