PepSep Four Columns have a length of 4 cm. As a shorter column, faster separation gradients can be used, yielding results up to three times faster than columns used in standard proteomics workflows. These columns would be an excellent choice for high-throughput applications.
PepSep Four Columns are available in different inner diameters. The column with a wider ID is a good choice to reduce backpressure, which can accelerate HPLC valve wear, overpressure HPLC systems and potentially cause column damage.
For smooth, safe, and easy connections to the high-pressure end of the column (often via transfer line) the columns are equipped with the patented ultra-high pressure (UHP) connection. The UHP connection is tested to be used up to 1000 bar.
On the columns low pressure end, the columns are equipped with the patented nanoConnect (nC) connector, ensuring a zero dead volume connection. By design, the nanoConnect system makes union and emitter connections quick, reliable, and straightforward.
| Stationary phase | C18 ReproSil AQ |
| Particle size | 1.9 µm |
| Pore size | 120 Å |
| Surface area | 300 m²/g |
| pH range | 2-8 |
| Length | 40 mm |
| Inner diameter | 50 or 150 µm |
| Column type | RP |
| Max. Temperature | 60 °C |
| Max. Pressure | 1000 bar |
| Inlet | UHP |
| Outlet |
nanoConnect |
